Research on: Ethosomal Drug Delivery System for White Flower Clitoria ternatea Leaf Extract: Formulation, Characterization, and Anti-inflammatory Evaluation

Abstract

This study evaluates the antioxidant and anti-inflammatory potential of Clitoria ternatea and its enhancement through an ethosomal gel formulation. Phytochemical and GC–MS analysis confirmed the presence of bioactive compounds. SEM revealed a porous structure with micro- to submicron-sized particles. The ethosomal gel showed significantly higher antioxidant and anti-inflammatory activity than the crude extract in both in vitro and in vivo studies. The improved efficacy is attributed to enhanced solubility, stability, and skin penetration. The formulation demonstrates promising potential for the topical treatment of inflammatory skin disorders

Materials and Methods: Clitoria ternatea extract was prepared and subjected to phytochemical and GC–MS analysis. Ethosomes were formulated and incorporated into a Carbopol gel. SEM analysis was performed to study morphology. Antioxidant and anti-inflammatory activities were evaluated using in vitro methods, followed by in vivo assessment using the formalin-induced paw edema model. Results: SEM showed a porous structure with particle sizes ranging from nanometers to micrometers. The ethosomal gel exhibited higher antioxidant and anti-inflammatory activity than the crude extract in a dose-dependent manner. In vivo studies confirmed significant and sustained anti-inflammatory effects comparable to those of the standard drug.

Conclusion: Ethosomal encapsulation significantly enhances the therapeutic efficacy of Clitoria ternatea extract. The developed gel is a promising candidate for topical anti-inflammatory applications.

Keywords: Clitoria ternatea, ethosomes, antioxidant, anti-inflammatory, SEM, and topical delivery.